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Mass spectrometric approaches for the analysis of phytosterols in biological samples.

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dc.contributor.authorGachumi, George N.
dc.contributor.authorEl-Aneed, Anas
dc.date.accessioned2019-06-05T19:41:16Z
dc.date.available2019-06-05T19:41:16Z
dc.date.issued2017-10-23
dc.description.abstractPlant sterols (phytosterols) are important structural components of plant cellular membranes and they play a major role during development and metabolism. They have health-associated benefits, especially in lowering blood cholesterol levels. Due to their many health claims, there is a growing interest in their analysis. Although various analytical strategies have been employed in analyzing phytosterols, chromatography linked to mass spectrometry (MS) is superior due to its sensitivity. Furthermore, specificity and selectivity are enhanced by utilizing tandem mass spectrometry (MS/MS). This article reviews the various mass spectrometric strategies used for the analysis of phytosterols. It highlights the applications and limitations associated with each MS strategy in various sample matrices such as plant, human, animal, food, and dietary supplements. GC-MS was historically the method of choice for analysis; however, the derivatization step rendered it tedious and time-consuming. On the other hand, liquid chromatography coupled to MS (LC-MS) simplifies the analysis. Many ionization techniques have been used namely electrospray ionization (ESI), atmospheric pressure chemical ionization (APCI) and atmospheric pressure photoionization (APPI). APCI showed superiority in terms of ion intensity and consistency in ion formation, primarily forming [M+H-H2O]+ ions rather than [M+H]+. In addition, matrix assisted laser desorption ionization (MALDI) as well as ambient mass spectrometry such as direct analysis in real time (DART) have also been evaluated.en_US
dc.description.sponsorshipAgriculture Development Fund grant, Ministry of Agriculture, Government of Saskatchewan, Canada.en_US
dc.description.versionPeer Revieweden_US
dc.identifier.citationGeorge N. Gachumi and Anas El-Aneed (2017), Mass Spectrometric Approaches for the Analysis of Phytosterols in Biological Samples. J Agric Food Chem. 2017 Nov 29;65 (47):10141-10156en_US
dc.identifier.doi10.1021/acs.jafc.7b03785
dc.identifier.pmid29058915
dc.identifier.urihttp://hdl.handle.net/10388/12113
dc.language.isoenen_US
dc.publisherACS Publicationsen_US
dc.rightsAttribution-NonCommercial-ShareAlike 2.5 Canada*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/2.5/ca/*
dc.subjectPhytosterols, GC-MS, plant sterols, LC-APCI-MS, LC-ESI-MSen_US
dc.titleMass spectrometric approaches for the analysis of phytosterols in biological samples.en_US
dc.typePostprinten_US

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