Repository logo
HARVEST
 

Development and validation of fast and simple flow injection analysis-tandem mass spectrometry (FIA-MS/MS) for the determination of metformin in dog serum

Simple item page
dc.contributor.authorEl-Aneed, Anas
dc.contributor.authorArnason, Terra
dc.contributor.authorMichel, Deborah
dc.contributor.authorGaunt, Matthew
dc.date.accessioned2016-12-16T17:03:31Z
dc.date.available2016-12-16T17:03:31Z
dc.date.issued2015-03-25
dc.description.abstractA simple, fast and sensitive quantification method for the drug metformin in dog serum was developed using flow injection analysis (FIA)- tandem mass spectrometry (MS/MS). The method was fully validated according to industry standards. It is the first time that FIA-MS/MS for metformin was developed surpassing all existing methods in terms of time of analysis. The quantification method was dependent on the formation of [M+H]+ using electrospray ionization (ESI) and employing multiple reaction monitoring (MRM) using quadrupole- linear ion trap (4000 QTRAP®) instrument. A deuterated internal standard (IS) of metformin bearing six deuterium atoms was used to compensate for matrix effects and for variation in ion current within the ESI source. The ion transitions that were monitored were m/z 130.1 -> m/z 71.0 and m/z 130.1-> m/z 60.1 for metformin and m/z 136.0 -> m/z 77.0 for the internal standard. A linear response (r = 0.9966) was established for a range of concentrations of 5- 2340 ng/ml. The inter- and intra-day variations were within the acceptable criteria for all quality control samples. The method was successfully applied for measurement of serum metformin concentration in dogs after intravenous injection.en_US
dc.description.sponsorshipCanada Foundation for Innovation; University of Saskatchewanen_US
dc.identifier.citationMichel D, Gaunt MC, Arnason T, El-Aneed A. J Pharm Biomed Anal. 2015 Mar 25 (107): 229-35. doi: 10.1016/j.jpba.2014.12.012en_US
dc.identifier.doi10.1016/j.jpba.2014.12.012
dc.identifier.pmid25618829
dc.identifier.urihttp://hdl.handle.net/10388/7623
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.rightsAttribution-NonCommercial-NoDerivs 2.5 Canada*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.5/ca/*
dc.subjectDog serum; Flow injection analysis; MS/MS; Metformin; Quantificationen_US
dc.titleDevelopment and validation of fast and simple flow injection analysis-tandem mass spectrometry (FIA-MS/MS) for the determination of metformin in dog serumen_US
dc.typePostprinten_US

Files

Original bundle
Now showing 1 - 2 of 2
Thumbnail Image
Name:
Michel et al 2015.pdf
Size:
415.5 KB
Format:
Adobe Portable Document Format
Description:
Main article
Download
Thumbnail Image
Name:
Michel 2015_Graphical Abstract.pdf
Size:
340.2 KB
Format:
Adobe Portable Document Format
Description:
Graphical Abstract
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
2.29 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Pharmacy