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Rapid and Simple Flow Injection Analysis-Tandem Mass Spectrometric (FIA-MS/MS) Method for the Quantification of Melphalan in Lipid-Based Drug Delivery System

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dc.contributor.authorMohammed-Saeid, Waleed
dc.contributor.authorMichel, Deborah
dc.contributor.authorEl-Aneed, Anas
dc.date.accessioned2019-04-29T21:47:23Z
dc.date.available2019-04-29T21:47:23Z
dc.date.issued2017-07-01
dc.descriptionNatural Sciences and Engineering Research Council of Canada (NSERC), Canada Foundation for Innovation (CFI)en_US
dc.description.abstractThe use of the anticancer drug melphalan is limited due to its poor water solubility. To address such limitation, it is incorporated within a novel delivery system using β-cyclodextrin-gemini surfactants (18:1βCDg). Herein, two fast and simple FIA-MS/MS methods are developed for the quantification of melphalan (Mel) within the drug delivery system so that the solubilization efficiency of the system can be assessed. FIA-MS/MS methods are developed using a triple quadrupole-linear ion trap mass spectrometer, equipped with electrospray ionization (ESI) in the positive ion mode. A deuterated form of melphalan (melphalan-d8) was used as an internal standard (IS). The methods were validated according to the FDA guidance. A linearity in the range of 2–100 ng/mL and accuracy and precision below 15% were observed for all standard points and quality control samples. The intra- and inter-day variations, freeze-thaw stability were within the acceptable range according to the criteria set by regulatory guidelines. On the other hand, other stability measures, such as room temperature stability and long term stability did not meet the required guidelines in some cases, indicating the need for quick sample analysis upon preparation. Such a fact could have been overlooked if full method validation was not performed. The developed methods were applied to determine the encapsulation/solubilisation of [18:1βCDg\Mel] delivery system. 18:1βCDg enhances the aqueous solubility of melphalan without the need for co-solvent. The highest melphalan solubility was observed at the 18:1βCDg\Mel complex molar ratio of 2:1. This study demonstrated that a fast analysis for the purpose of quantifying a chemically unstable drug, such as melphalan is feasible and important for the development of commercial dosage forms.en_US
dc.description.versionPeer Revieweden_US
dc.identifier.citationWaleed Mohammed-Saeid, Deborah Michel, Ildiko Badea, Anas El-Aneed. (2017), Rapid and Simple Flow Injection Analysis-Tandem Mass Spectrometric (FIA-MS/MS) Method for the Quantification of Melphalan in Lipid-Based Drug Delivery System. Rapid Commun. Mass Spectrom., 31(18): 1481-1490en_US
dc.identifier.doi10.1002/rcm.7926
dc.identifier.pmid28667829
dc.identifier.urihttp://hdl.handle.net/10388/11991
dc.language.isoenen_US
dc.publisherWileyen_US
dc.rightsAttribution-NonCommercial-ShareAlike 2.5 Canada*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/2.5/ca/*
dc.subjectFlow Injection Analysisen_US
dc.subjectMass Spectrometry MSMSen_US
dc.subjectMelphalan, β-cyclodextrinen_US
dc.subjectDrug Delivery  en_US
dc.titleRapid and Simple Flow Injection Analysis-Tandem Mass Spectrometric (FIA-MS/MS) Method for the Quantification of Melphalan in Lipid-Based Drug Delivery Systemen_US
dc.typePostprinten_US

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