Repository logo
HARVEST
 

Seasonal dynamics of methanotrophic bacteria in a boreal oil sands end-pit lake

Simple item page
dc.contributor.authorAlbakistani, Emad
dc.contributor.authorNwosu, Felix Chinweije
dc.contributor.authorFurgason, Biegler, Chantel
dc.contributor.authorHaupt, Evan S.
dc.contributor.authorSmirnova, Angela V.
dc.contributor.authorVerbeke, Tobin
dc.contributor.authorLee, Eun-Suk
dc.contributor.authorKim, Joong-Jae
dc.contributor.authorChan, Amelia
dc.contributor.authorRuhl, Ilona A.
dc.contributor.authorSheremet, Andriy
dc.contributor.authorRudderham, Sarah B.
dc.contributor.authorLindsay, Matthew B. J.
dc.contributor.authorDunfield, Peter
dc.date.accessioned2022-09-27T20:35:17Z
dc.date.available2022-09-27T20:35:17Z
dc.date.issued2021-11-24
dc.descriptionCopyright © 2022 American Society for Microbiology. All Rights Reserved.en_US
dc.description.abstractBase Mine Lake (BML) is the first full-scale demonstration end pit lake for the oil sands mining industry in Canada. We examined aerobic methanotrophic bacteria over all seasons for 5 years in this dimictic lake. Methanotrophs comprised up to 58% of all bacterial reads in 16S rRNA gene amplicon sequencing analyses (median 2.8%), and up to 2.7 × 104 cells mL−1 of water (median 0.5 × 103) based on qPCR of pmoA genes. Methanotrophic activity and populations in the lake water were highest during fall turnover and remained high through the winter ice-covered period into spring turnover. They declined during summer stratification, especially in the epilimnion. Three methanotroph genera (Methylobacter, Methylovulum, and Methyloparacoccus) cycled seasonally, based on both relative and absolute abundance measurements. Methylobacter and Methylovulum populations peaked in winter/spring, when methane oxidation activity was psychrophilic. Methyloparacoccus populations increased in the water column through summer and fall, when methane oxidation was mesophilic, and also predominated in the underlying tailings sediment. Other, less abundant genera grew primarily during summer, possibly due to distinct CH4/O2 microniches created during thermal stratification. These data are consistent with temporal and spatial niche differentiation based on temperature, CH4 and O2. This pit lake displays methane cycling and methanotroph population dynamics similar to natural boreal lakes.en_US
dc.description.sponsorshipNSERC CRD (CRDPJ478071-14); King Abdullah Scholarship Program (KASP) - Ministry of Education, Kingdom of Saudi Arabia; NSERC CGS-M; NSERC CRD (CRDPJ476388–14).en_US
dc.description.versionPeer Revieweden_US
dc.identifier.citationAlbakistani, E.A., Nwosu, F. C., Furgason, C., Haupt, E. S., Smirnova, A. V., Verbeke, T. J., Lee, E.-S., Kim, J.-J., Chan, A., Ruhl, I. A., Sheremet, A., Rudderham, S. B., Lindsay, M. B. J. & Dunfield, P. F. (2022). Seasonal dynamics of methanotrophic bacteria in a boreal oil sands end pit lake. Applied and Environmental Microbiology, 88(3), e0145521. https://doi.org/10.1128/aem.01455-21en_US
dc.identifier.doi10.1128/aem.01455-21
dc.identifier.urihttps://hdl.handle.net/10388/14233
dc.language.isoenen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.subjectmethaneen_US
dc.subjectmethanotrophen_US
dc.subjectstratified lakeen_US
dc.subjectdimictic lakeen_US
dc.subjectland reclamationen_US
dc.subjectend pit lakeen_US
dc.subjectmethanotrophsen_US
dc.titleSeasonal dynamics of methanotrophic bacteria in a boreal oil sands end-pit lakeen_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Albakistani et al. (2022) Applied and Enviro. Microbiol.pdf
Size:
3.07 MB
Format:
Adobe Portable Document Format
Description:
Main article
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
2.28 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Geological Sciences