Agriculture

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https://hdl.handle.net/10388/14353

Projections show that food production must double by 2050 to feed the world's growing population. We are working to strengthen Saskatchewan's agricultural leadership with new science, technology and policies to help feed a hungry world adequately, safely and sustainably.

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Investigation of polyphenol diversity among lentil species (Lens spp.) using mass spectrometry-based metabolomics guided by photodiode array detection
(ScienceDirect, 2025-03) Purves, Randy W.; Khazaei, Hamid; Elessawy, Fatma M.; Munro, Roger; Shurmer, Bryn O.; Vandenberg, Albert
Polyphenol diversity was investigated among seven lentil species, including Lens culinaris (cultivated lentil), L. orientalis, L. tomentosus, L. odemensis, L. lamottei, L. ervoides, and L. nigricans, using photodiode array detection coupled with liquid chromatography - mass spectrometry (LC-MS). Principal component analysis showed that most species grouped individually, except L. tomentosus and L. odemensis, which overlapped. The LC-MS data from both negative and positive electrospray ionization modes were used to identify 85 polyphenols observed in the UV–vis spectra, which included 27 proanthocyanidins, 17 flavonols, 15 flavones, and 12 hydroxybenzoic acids. An untargeted (comprehensive) analysis of the LC-MS data using Compound Discoverer software identified additional polyphenols (231 total), including numerous overlapping proanthocyanidins that contribute to a broad peak in the UV–vis spectra. The software analysis uncovered some notable differences among polyphenol profiles and intensities within the flavones, flavonols, and phenolic acids present in the species. This result indicates natural variation among the lentil wild relatives, which in part, is attributed to structurally isomeric compounds. A hierarchical clustering analysis, and a differential analysis using volcano plots used to look for statistically significant differences in polyphenols, illustrated significantly lower relative levels of polyphenols in L. culinaris compared with the wild types, especially within the proanthocyanidins and flavones. Our results highlight the potential of lentil wild relatives to enhance lentil seed quality.
Structural and functional analysis of a bile salt hydrolase from the bison microbiome
(Elsevier, 2024-10) Asar, Radwa; Dhindwal, Poonam; Ruzzini, Antonio
The bile salt hydrolases (BSHs) are significant constituents of animal microbiomes. An evolving appreciation of their roles in health and disease has established them as targets of pharmacological inhibition. These bacterial enzymes belong to the N-terminal nucleophile superfamily and are best known to catalyze the deconjugation of glycine or taurine from bile salts to release bile acid substrates for transformation and or metabolism in the gastrointestinal tract. Here, we identify and describe the BSH from a common member of the Plains bison microbiome, Arthrobacter citreus (BSHAc). Steady-state kinetic analyses demonstrated that BSHAc is a broad-spectrum hydrolase with a preference for glycine-conjugates and deoxycholic acid (DCA). Second-order rate constants (kcat/KM) for BSHAc-catalyzed reactions of relevant bile salts—glyco- and tauro-conjugates of cholic acid and DCA— varied by ∼30-fold and measured between 1.4 × 105 and 4.3 × 106 M-1s-1. Interestingly, a pan-BSH inhibitor named AAA-10 acted as a slow irreversible inhibitor of BSHAc with a rate of inactivation (kinact) of ∼2 h-1 and a second order rate constant (kinact/KI) of ∼24 M-1s-1 for the process. Structural characterization of BSHAc reacted with AAA-10 showed covalent modification of the N-terminal cysteine nucleophile, providing molecular details for an enzyme-stabilized product formed from this mechanism-based inhibitor’s α-fluoromethyl ketone warhead. Structural comparison of the BSHs and BSH:inhibitor complexes highlighted the plasticity of the steroid-binding site, including a flexible loop that is variable across well-studied BSHs.
Insight into antimicrobial resistance at a new beef cattle feedlot in western Canada
(AMS Journals, 2023-10) Kos, Daniel; Schreiner, Brittany; Thiessen, Stuart; McAllister, Tim; Jelinski, Murray; Ruzzini, Antonio
In North America, beef production relies on the administration of antimicrobials to manage disease. Bovine respiratory disease (BRD) is the most significant disease of beef cattle, and antimicrobial resistance (AMR) to conventional therapies presents an existential risk to animal welfare and food production. While AMR surveillance programs are poised to help facilitate antimicrobial stewardship and decision making at feedlots, monitoring strategies for large numbers of animals at an individual or group level are time consuming and costly. Accordingly, we completed a pilot investigation of feedlot water bowls, which is an understudied interface between cattle and bacteria. By performing cultivation-dependent and cultivation-independent studies, we demonstrate that water bowl-dwelling bacteria can act as sentinel organisms for clinically relevant antimicrobial resistance genes (ARGs) and that cattle have an impact on the microbial communities in the bowls. Moreover, by sampling water at a feedlot site before animal arrival, we detected resistance to two antibiotics: florfenicol and tulathromycin. After just 4 weeks of operation, multidrug-resistant bacteria were routinely found in most water bowls. A comparison of ARGs encoded by five water bowl bacterial isolates along with previously reported source and wastewater metagenomes to those found in BRD pathogens confirmed the utility of using water samples for AMR surveillance.
A neglected and emerging antimicrobial resistance gene encodes for a serine-dependent macrolide esterase
(PNAS, 2023-02) Dhindwal, Poonam; Thompson, Charis; Kos, Daniel; Planedin, Koa; Jain, Richa; Jelinski, Murray; Ruzzini, Antonio
The accumulation of antimicrobial resistance genes (ARGs) in specific environments, and eventually in pathogens, challenges the utility of antibiotics. Thousands of ARGs and resistance-conferring mutations are known and accessible in curated databases. Nevertheless, our catalog of ARGs is incomplete. Blind spots—novel and unreported ARGs—pose unknown risks to human health, animal wellness, and the sustainability of agriculture. A novel and unreported ARG is one that cannot be identified by homology-based inference. These unknown genes are absent from the ARG databases that serve antimicrobial resistance (AMR) surveillance programs intended to inform clinical practice. Growing concerns related to antimicrobial use and efficacy prompted us to evaluate watering bowls as sentinel systems for ARG detection at a beef cattle feedlot. The identification and functional characterization of a neglected ARG that resulted form the basis of this report.
Retrospective analysis of antimicrobial resistance associated with bovine respiratory disease
(ASM Journals, 2025-02) Kos, Daniel; Jelinski, Murray; Ruzzini, Antonio
The administration and utility of antibiotics to control and treat bovine respiratory disease (BRD) in beef cattle feedlots is a growing concern. Antimicrobial resistance (AMR) among BRD-associated bacterial pathogens has been the subject of cultivation-dependent and cultivation-independent surveillance. Bacterial genome sequencing and metagenomic approaches facilitate the characterization of AMR in the beef industry; however, the current collection of cattle-associated AMR research programs lack connections to each other. A more integrated view of how antimicrobial use (AMU) is related to resistance at a gene level is needed. We sought to establish a catalog of commonly observed AMR genes (ARGs) in opportunistic bacterial pathogens that contribute to BRD using publicly available data sets that were generated by the scientific community with and without AMU in mind. The presence of these clinically relevant ARGs appeared to differ by geography. Greater sampling in North America facilitated the generation of a list of ARGs often encoded by Mannheimia haemolytica and Pasteurella multocida. Detection of clinically relevant ARGs in shotgun metagenomes of cattle-associated and accessible feedlot samples such as water, soil, and feces was possible but limited by relative sequence read abundance. An exception was the tylosin esterase-encoding gene estT, which is among the most frequently observed ARGs in M. haemolytica and feedlot-related metagenomic data sets. Finally, by re-evaluating studies on the impact of AMU on AMR in beef production systems, we show that conventional practices, including in-feed antibiotic use, increase the relative abundance of ARGs in animal-derived samples.
Recycling Olive Mill Wastewater to Calcareous Soil: Effect of Preplanning Application Period on Phytotoxicity, Corn Growth, and Nutrient Uptake
(MDPI, 2025-02) Hasan, Yousef N. Y.; Ahmed, Ibrahim; Al-Barakah, Fahad; Schoenau, Jeffrey; Alotaibi, Khaled D.
This study investigated the effects of applying olive mill wastewater (OMWW) at different periods prior to corn (Zea mays) sowing on germination rate (GR), growth, and soil nutrient availability in calcareous soil. The OMWW was applied at rates of 0, 20, 40, and 60 m3 ha−1 and was allowed to remain in soil for zero, one, two, three, or four months before sowing corn seeds. Immediate planting after OMWW application significantly reduced the GR, with rates of 83%, 75%, and 63% at 20, 40, and 60 m3 ha−1, respectively. Germination improved when corn was sown one month after OMWW application, with a GR of 92% at both 20 and 40 m3 ha−1 and 79% at 60 m3 ha−1. The GR increased to 96% for the 40 and 60 m3 ha−1 rates when OMWW was applied two months before planting. The adverse impact on GR disappeared when OMWW was in the soil for three months before sowing, providing a GR similar to the unamended control. Corn dry matter yield also improved when OMWW was applied two to three months before planting. The phytotoxic effects of OMWW, due to its high polyphenol content, diminished over time due to rapid degradation in calcareous soils. Soil available N and P were highest, and plant N, P, and K content increased, when OMWW was applied two months prior to planting. Soil pH decreased from 7.8 to 7.2 at 60 m3 ha−1 of OMWW at planting time. Results suggest that OMWW can enhance soil quality and corn growth if applied one to two months before planting to avoid possible negative impact on germination. This work bridges the gap between waste management and sustainable agriculture, offering practical guidelines for OMWW utilization.
Bacterial diversity and community structure in native legume root nodules in intact and mining-disturbed Arctic tundra
(Canadian Science Publishing, 2025-02) Li, Angie S; Standen, Katherine Marie; Stewart, Katherine J; Lamb, Eric
Mining restoration in Arctic regions is challenging due to harsh environmental conditions with slow natural ecosystem recovery taking decades or even centuries. Identifying native species that can both colonize disturbed areas and contribute to soil development is critical to hasten restoration timelines. Nodules were collected from four native legume species (Astragalus alpinus Linnaeus, Hedysarum americanum (Michaux ex Pursh) Britton, Oxytropis arctica R. Brown, and Oxytropis maydelliana Trautvetter) observed to be naturally colonizing gravel quarries within a mine footprint near Rankin Inlet, Nunavut, Canada. Samples came from both gravel quarries and adjacent intact tundra. Next-generation sequencing of the 16S and nifH regions was used to characterize the nodule bacterial community composition and diversity. Despite the large differences in soil conditions between gravel substrates and intact tundra, no significant effects of soil environment were found on bacterial community composition within plant nodules. Further, few differences were observed in nodule communities between the plant species. Overall, the study suggests that the microbial propagules necessary for successful nodulation are present in gravel quarries. While restoration efforts involving native legumes may succeed without commercial inoculants, further research is needed to determine whether the rhizobia in these environments can provide sufficient nitrogen to support robust host plant growth.
Microwave-infrared drying of cannabis (Cannabis sativa L.): Effect on drying characteristics, energy consumption and quality
(Elsevier, 2024-02) Das, Pabitra Chandra; Baik, Oon-Doo; Tabil, Lope G.
Conventional cannabis drying is time-consuming and energy intensive. A quick and dependable drying of cannabis is essential to ensure high-quality products to meet increasing demands. This study explored a combined microwave and infrared (MI) drying on cannabis comparing with control environmental (CE) drying. MI was very efficient with a short drying time of 16–200 min, high moisture diffusivity of 7.95×10−09-8.70×10−08 m2/s, and low energy consumption of 390.49–1611.42 kJ. The Page and Modified Page drying models fitted well to describe and predict the MI drying characteristics of cannabis. Microstructural images identified shrinkage in glandular trichomes of cannabis, whereas colorimetric assessment resulted in alteration of color attributes due to MI drying. It also facilitated the conversion of acidic cannabinoids to their neutral forms by decreasing tetrahydrocannabinolic acid (THCA) in g/g of dry matter from 20.15% to 7.57% and increasing tetrahydrocannabinol (THC) from 6.31% to 16.65% that insignificantly (p≥0.05) affected the total THC level. MI drying resulted in a total terpenes concentration (%g/g of dry matters) of 0.541–0.730, insignificantly lower than CE drying (0.768). Overall, the study highlights MI as a rapid and energy-efficient drying for obtaining high quality cannabis, particularly for medicinal applications.
Postharvest Operations of Cannabis and Their Effect on Cannabinoid Content: A Review
(MDPI, 2022-08) Das, Pabitra Chandra; Vista, Alec Roger; Tabil, Lope; Baik, Oon-Doo
In recent years, cannabis (Cannabis sativa L.) has been legalized by many countries for production, processing, and use considering its tremendous medical and industrial applications. Cannabis contains more than a hundred biomolecules (cannabinoids) which have the potentiality to cure different chronic diseases. After harvesting, cannabis undergoes different postharvest operations including drying, curing, storage, etc. Presently, the cannabis industry relies on different traditional postharvest operations, which may result in an inconsistent quality of products. In this review, we aimed to describe the biosynthesis process of major cannabinoids, postharvest operations used by the cannabis industry, and the consequences of postharvest operations on the cannabinoid profile. As drying is the most important post-harvest operation of cannabis, the attributes associated with drying (water activity, equilibrium moisture content, sorption isotherms, etc.) and the significance of novel pre-treatments (microwave heating, cold plasma, ultrasound, pulse electric, irradiation, etc.) for improvement of the process are thoroughly discussed. Additionally, other operations, such as trimming, curing, packaging and storage, are discussed, and the effect of the different postharvest operations on the cannabinoid yield is summarized. A critical investigation of the factors involved in each postharvest operation is indeed key for obtaining quality products and for the sustainable development of the cannabis industry.
Enhancing drying efficiency and terpene retention of cannabis using cold plasma pretreatment
(ScienceDirect, 2025-11) Das, Pabitra Chandra; HEYDARI FOROUSHANI, MOHAMAD MEHDI; Baik, Oon-Doo; Zhang, Lifeng; Tabil, Lope
Hang-drying of cannabis at room conditions is a slow process and leads to the risk of microbial growth. This method can sometimes prevent cannabis from reaching the equilibrium moisture content (EMC) below the safe storage threshold. On the other hand, high-temperature drying techniques are faster but negatively impact the secondary metabolites. Cold plasma (CP) is a novel technique explored in this study to treat cannabis at various operational conditions of plasma jet (power: 300, 350, and 400 W, time: 20, 30, and 40 s) prior to drying at environmental conditions of 25°C and 50 % RH. The findings revealed that untreated cannabis samples reached an equilibrium moisture content (EMC) of approximately 16 % in 1260 min. In contrast, CP-pretreated samples achieved lower EMCs of 10–14 % within 690–840 min. CP pretreatment also resulted in high moisture diffusivity, lower energy consumption, and higher energy efficiency. Increasing CP power and residence time accelerated the decarboxylation of cannabinoids, leading to the formation of more tetrahydrocannabinol (THC) and less tetrahydrocannabinolic acid (THCA), without significantly affecting the total THC (27.45 % untreated vs. 25.82 % - 28.36 % g/ g of dry matter in CP pretreated samples). Compared with untreated dried inflorescences, the 400 W and 30 s CP treated inflorescences resulted in the retention of 96 % of terpenes, whereas all 300 W CP treated samples retained > 90 % of terpenes. Overall, the study highlights that CP pretreatment is a promising technology for the cannabis industry in shortening the drying time and preserving the product quality, especially terpenes.
Melanin from the fungus Gliocephalotrichum simplex protects seeds from the effects of exposure to gamma radiation
(Nature, 2025) Kunal, Swaraj; Bhanushali, Sushrut; Raghukumar, Seshagiri; Dadachova, Ekaterina
Protection of seeds from ionizing radiation is an important future need in space travel. We examined the usefulness of eumelanin from the fungus Gliocephalotrichum MTCC 5489 for this purpose. Seeds of Oryza sativa, Brassica nigra, Vigna radiata and V. aconitifolia were enclosed in Petri dishes coated with paint containing various concentrations of nanomelanin and exposed to doses of 0.1 to 2.0 kGy Cs-137 or 0.1 to 0.5 kGy Co-60 radiation. While Cs-137 radiation severely affected the rate of germination (germination index, GI) and germination percentage of Oryza sativa, whereas other seeds were less affected, Co-60 markedly diminished the GI of all the seeds. Increasing concentrations of melanin coating afforded significant protection to the GI of O. sativa seeds exposed to Cs-137 and to all seeds exposed to Co-60 radiation. The germination percentage of seeds was dose- and radiation dependent, with no effect observed for all doses of Cs-137 or 0.1 kGy Co-60 radiation, whereas 0.5 kGy Co-60 radiation killed 50 to 60% of the unprotected seeds. Melanin paint offered nearly 100% survival. This study demonstrated that melanin-containing materials can provide effective shielding from gamma radiation for diverse types of seeds that are important for human consumption, which has implications for space agriculture and agriculture in extreme environments.
Balance sheet strength in the oil and gas industry: Saving for a rainy day or making hay while the sun shines
(Advances in Accounting, 2025-06) Anderson, Mark; Ma, Yan; Park, Han-Up
We examine how a strategic emphasis on balance sheet strength relates to investment decisions and performance over time for firms operating in a cyclical environment. From a series of discussions with industry insiders and readings of disclosures for prominent oil and gas (O&G) companies in Canada, we identify two groups of upstream O&G firms based on how they match their resources and capabilities with the uncertainties posed by industry economic cycles. One group of firms borrows and invests aggressively when oil prices are strong and funds are available – “making hay while the sun shines”, while the other group grows conservatively to build and maintain balance sheet strength – “saving for a rainy day”. We use average cash flows to debt for each firm over time to measure emphasis on balance sheet strength and separate firms into rainy day and making hay companies. We leverage two steep price declines to observe the behavior of firms over industry cycles: one triggered by the widespread 2008 financial crisis and the other by a distinct and prolonged O&G industry downturn in 2014. While investment declined generally in both cases, we find that the decline in investment was significantly less for rainy day companies than making hay firms after the 2014 downturn. Across time, we find that rainy day companies make shrewder acquisitions and operate more efficiently than making hay companies. Nonetheless, the capital market rewards making hay companies with higher market valuation, but this is reduced in downturns.
Oxidative Stability of Novel Peptides (Linusorbs) in Flaxseed Meal-Fortified Gluten-Free Bread
(Foods, 2025-01) Shim, Youn Young; Burnett, Peta-Gaye G.; Olivia, Clara M.; Zou, Xianguo; Lee, Sung Jin; Kim, Hye-Jin; Kim, Young Jun; Reaney, Martin
Flaxseed meal, rich in water-soluble gums, improves the texture of gluten-free (GF) products. Bioactive antioxidant peptides from flaxseed, known as linusorbs (LOs) or cyclolinopeptides, may provide health benefits. However, the stability of flaxseed-derived LOs during dough preparation, baking, and storage remains unclear. To investigate this, GF bread dough and bread were prepared with flaxseed meal, and the LO content was determined in the flaxseed meal, the bread flour with the flaxseed meal, the dough, and the bread. The LO levels were also monitored during storage at various temperatures (−18 °C, 4 °C, and 22–23 °C) for 0, 1, 2, and 4 weeks using high-performance liquid chromatography–diode array detection (HPLC-DAD). The levels of oxidized LOs, such as [1–9-NαC],[1-(Rs,Ss)-MetO]-linusorb B2 (LO14), remained relatively stable in the flaxseed meal and the flour derived from it across under all conditions for up to 4 weeks. Due to microbial contamination, the dough could not be stored at either 4 or 21 °C, and the bread could only be stored at 21 °C for one week. However, the bread and dough could be stored for up to 4 weeks at −18 °C, and the bread at 4 °C, without a significant loss of LOs. The main changes in LOs occurred during processing rather than storage. Reduced LOs were found in higher concentrations in the flour and meal compared to the dough and bread, without a corresponding increase in oxidized LOs. The flaxseed meal-fortified bread maintained oxidative stability when stored at low temperatures. This is the first study to investigate the effect of baking conditions on LO content and antioxidant properties.
A multiplex PCR assay for the detection of six foliar fungal pathogens of faba bean
(Canadian Journal of Plant Pathology, 2025-02) Bruce, Margaret; Stratford, Ethan; Armstrong-Cho, Cheryl; Banniza, Sabine
ral foliar fungal diseases: ascochyta blight (Ascochyta fabae), alternaria leaf spot (Alternaria sp.), chocolate spot (Botrytis cinerea and Botrytis fabae), anthracnose (Colletotrichum lentis) and stemphylium blight (Stemphylium spp.). Under conducive conditions these diseases can cause significant economic losses. Visual diagnosis based on the appearance of lesions on faba beans is problematic, especially as they progress. The purpose of this study was to develop a multiplex polymerase chain reaction assay to detect the causal organisms of these diseases. Five pairs of primers, AFF7/AFR7 for A. fabae, BCF1/BCR1 for B. cinerea, BFF2/BFR2 for B. fabae, ClF2/ClR2 for C. lentis and Stem_F_S/Stem_R_S for Stemphylium spp. were developed. The previously developed primer pair aagpf1/aagpr1 was used to detect Alternaria sp. The primers were tested for specificity to their target pathogen and primer pairs AFF6/AFR6, aagpf1/aagpr1, BCF1/BCR1, BFF2/BFR2 and ClF2/ClR2 identified their target pathogens. The primer pair Stem_F_S/Stem_R_S identified S. beticola, S. botryosum, S. eturmiunum and S. vesicarium. Identification of pathogens with primers was evaluated using DNA from mycelia and from infected faba bean leaves generated through artificial inoculations under controlled conditions and collected from the field. A multiplex PCR assay with six primer pairs allowed for detection of the target pathogens to the species level for five pathogens, and to the genus level for species causing stemphylium blight. This assay enables quick diagnosis of leaf spots on faba bean, and minimizing time and effort needed to identify the primary cause of the infection through traditional isolation procedures.
Gene co-expression analysis reveals conserved and distinct gene networks between resistant and susceptible Lens ervoides challenged by hemibiotrophic and necrotrophic pathogens
(Scientific Reports, 2024-10) Cao, Zhe; Banniza, Sabine
As field crops are likely to be challenged by multiple pathogens during their development, the investigation of broad-spectrum resistance in the host is of great interest for crop genetic enhancement. In this study, we attempted to address this question by adopting a weighed gene co-expression approach to study the temporal transcriptome dynamics of resistant and susceptible recombinant inbred lines (RILs) derived from an intraspecific Len ervoides cross during the infection process with either the necrotrophic pathogens Ascochyta lentis or Stemphylium botryosum, or the hemibiotrophic pathogen Colletotrichum lentis. By comparing networks of resistant and susceptible RILs, seven network module pairs were found to possess high correlation coefficients (R > 0.70) and large number of overlapping genes (n > 100). The conserved co-regulation of genes in these network module pairs were involved in plant cell wall synthesis, cell division, cytoskeleton organization, and protein ubiquitin related processes and appeared to be common disease responses against these pathogens. On the other hand, we also identified eight modules with low correlation between resistance and susceptibility networks. Among those, a stronger gene co-expression in R-genes and small RNA processes in the resistant hosts may be enhancing L. ervoides resistance against A. lentis, C. lentis, and S. botryosum, whereas the higher level of synergistic regulation in the synthesis of arginine and glutamine and phospholipid and glycerophospholipids in the susceptible hosts may contribute to increased susceptibility in L. ervoides.
Electrostatic Particle Ionization for Reduction in Livestock and Potash Dust
(MDPI, 2025-01-15) Martel, Myra; Taylor, Matthew; Kirychuk, Shelley; Choi, Kwangseok; Guo, Huiqing; Zhang, Lifeng
Airborne dust is an important contaminant affecting the health and the environment, and a crucial concern in many workplaces such as animal facilities and potash mines. One of the techniques used for dust control is electrostatic particle ionization (EPI). This technology has been proven effective in reducing airborne dust; however, it has downsides, such as the generation of ozone and corrosion of electrodes. Thus, this study tested a corrosion-resistant carbon-fiber discharge electrode and compared it with electrodes commonly used in EPI systems, that is, stainless-steel and tungsten electrodes, in terms of collection efficiency for potash dust and wheat flour (representative of livestock dust), ozone production, and power consumption. The carbon-fiber electrode performed comparably to stainless-steel electrodes, particularly for potash dust, and performed better than the tungsten electrode in terms of dust collection efficiency. Moreover, it had the lowest energy consumption and generated the least amount of ozone. However, because of the limitations of this study (e.g., fewer samples, low air velocity, controlled conditions, and the use of wheat flour instead of livestock dust), tests under real barn or mining conditions are necessary to confirm the results.
Suspected fenugreek (Trigonella foenum-graecum L.) toxicosis in a herd of Saskatchewan beef cattle
(Sage Publications, 2025-01-09) Cowan, Vanessa; Koziy, Roman; Zemlak, Laurie; Campbell, John R.
An apparent outbreak of fenugreek forage toxicosis occurred in a beef cattle herd near Moose Jaw, Saskatchewan in February–May 2022. The herd had consumed fenugreek hay from late fall to early winter. Clinical signs included various degrees of weakness, ataxia, knuckling, walking on hocks, and recumbency. All adult cattle in the herd eventually died or were euthanized. Feed analysis did not reveal nutritional deficiencies or mycotoxin contamination. Liver mineral and vitamin status of affected animals did not indicate any consistent abnormality. The last live cow in the herd was presented to a veterinary teaching hospital for evaluation and subsequent postmortem examination. Major postmortem findings included emaciation, and sciatic nerve and spinal cord axonal degeneration. Histologic examination of the sciatic nerve showed Wallerian-like axonal degeneration, increased Schwann cell nuclei, and endoneurial fibrosis. Histologic examination of the spinal cord showed infrequent myelin sheath dilation and digestion chambers within white matter. These results are consistent with other reports of natural and experimental outbreaks of fenugreek poisoning in livestock. To our knowledge, fenugreek toxicosis has not been reported previously in Canada. We conclude that caution should be taken when feeding fenugreek hay to cattle.
Short rotation willow to restore degraded marginal land and enhance climate resiliency within the Prairie Pothole Region: A potential nature-based solution
(Elsevier, 2024-05-19) Shahariar, Shayeb; Soolanayakanahally, Raju; Bedard-Haughn, Angela
Short rotation willow (SRW) is a land management strategy involving the cultivation of rapidly growing, biomass-rich herbaceous-woody plants. This practice holds promise for renewable energy production, water quality preservation, carbon sequestration, greenhouse gas (GHG) mitigation, enhancement of soil extracellular enzyme activities (EEAs), and promotion of overall soil health. The rapid growth of SRW demands substantial water and nutrient resources, posing concerns when cultivated in marginal riparian lands within the Prairie Pothole Region (PPR), potentially leading to alterations in groundwater table (GWT) depth fluctuations, elevated soil salinity levels, and disruptions to biogeochemical cycles. Hence, this study comprehensively evaluated the effects of establishing SRW as a degraded marginal riparian land use practice in the PPR and attempted to answer several vital questions in the field and microcosm scale on soil hydrology, salinity, nutrients, soil organic carbon (SOC), GHG emissions, and EEAs involved in biogeochemical cycling. In a field experiment, the effects of SRW were evaluated by measuring the depth to GWT, groundwater and soil electrical conductivity (EC), macronutrients (N, P, K, and S), and SOC content in different fractions and chemical compositions during the first rotation (3-year cycle) compared with adjacent annual crop and pasture in two semi-arid PPR sites. In a microcosm experiment, GHG (CO2, CH4, and N2O) emissions and EEAs [β-glucosidase (BG), N-acetyl glucosaminidase (NAG), and alkaline phosphatase (AP)] were measured in intact soil cores treated with declining water tables and different groundwater salinity levels. No consistent land use impacts on GWT or soil EC were observed between sites. Land use in site B significantly impacted GWT depth, implying site-specific factors, such as topography and soil characteristics, may be dominant over land use effects. Under SRW, the levels of macronutrients in the soil varied but did not significantly reduce the overall nutrient content of the soil. Total SOC was highest in pasture; light fraction organic carbon and particulate organic carbon followed a similar land use pattern, i.e., pasture > SRW = annual crop. Land uses affected GHG emissions significantly in the order of pasture > annual crop = SRW. GHG emission varied with salinity and GWT but there was no interaction with land use practices. Soil EEAs were significantly impacted by different land uses, i.e., pasture > annual crop = SRW, suggesting that the effects resulted from associated SOC. Our microcosm experiment suggests that the SRW land use practice holds promise as a sustainable Nature-Based Solution for enhancing climate resiliency in PPR. It exhibits a lower global warming potential compared to annual crop and pasture. Therefore, widespread implementation of the SRW land use practice in degraded marginal land could help mitigate the effects of climate change in the region.
An experimental approach to farmer valuation of African rice genetic resources
(Wiley, 2024-07-04) Tyack, Nicholas; Arouna, Aminou; ABOUDOU, Rachidi; Ndjiondjop, Marie Noelle
Genebanks serve as both providers of valuable traits for breeding programs and repositories of diverse crop genetic material representing society's agricultural heritage. In this study, we use a Becker-DeGroot-Marschak mechanism to elicit the willingness-to-pay of rice farmers in Côte d'Ivoire for small amounts of African rice (Oryza glaberrima) landraces held by the genebank of the Rice Biodiversity Center for Africa, and for seed of newly developed ARICA rice varieties bred using genebank materials. Using a field experiment, we additionally investigate how randomized exposure to and experimentation with small amounts of African rice landrace seed or seed of advanced rice varieties developed by AfricaRice affect how smallholder rice farmers value these novel genetic resources. Surprisingly, we find that farmers generally value having access to African rice landraces at approximately the same level as for advanced rice varieties (and far above market rates for improved seed), and that those farmers who grew landrace seed in the offseason were willing to pay more than those who did not. Our results demonstrate the additional value provided by the conservation of African rice landrace varieties (apart from their use in breeding) and highlight the importance of experimentation in the adoption process.
Phosphorus fertilizer responsive bacteria and fungi in canola (Brassica napus L.) roots are correlated with plant performance
(Plant and Soil, 2025-02-17) Liu, Mengying; Mooleki, S. Patrick; Li, Yunliang; Schneider, Dave; Kochian, Leon V; Helgason, Bobbi
Background Canola (Brassica napus L.) has high phosphorus demand, but its seedlings are sensitive to seed-placed phosphorus fertilizers. Optimizing phosphorus fertilizer management for canola is critical and can benefit from insights into the root-associated microbiota, which enhances phosphorus availability through mineralization and solubilization. Methods We conducted a two-year field experiment applying monoammonium phosphate fertilizer at three rates (no addition, recommended rate, and high rate at 0, 17, and 32 kg P ha−1 year−1) using two opener placements (narrow at 2.5 cm vs. wide at 10 cm). Canola performance was evaluated, and rhizosphere and root bacterial and fungal microbiota was profiled by DNA amplicon sequencing. Results High-rate and near-seed placement of phosphorus (32 kg P ha−1 in the 2.5 cm opener) reduced canola seedling emergence but not biomass or yield, which were higher in 2020 than in 2019. Yearly variations and plant growth stages impacted the rhizosphere and root microbiota, while phosphorus fertilization only affected the root microbiota. Phosphorus fertilization enriched Burkholderia-Caballeronia-Paraburkholderia, Luteibacter, Amaurodon, Trichoderma, and Penicillium in roots, Conversely, Chryseobacterium, Chitinophaga, Flavobacterium and Olpidium were more prevalent in roots without phosphorus addition. Yield positively correlated with the relative abundance of Burkholderia-Caballeronia-Paraburkholderia and Trichoderma in roots. Conclusions Phosphate fertilizer rates and placements affected canola germination but not yield. Profiling of phosphorus-responsive root microbes suggests that phosphate fertilizer rate and placement at seeding can have a lasting impact on the canola root microbiota as the plant matures, modulating plant growth responses to soil phosphorus availability.

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