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      Redox Potential-Controlled 1,3-Propanediol Production from Glycerol by Lactobacillus panis PM1

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      WU-THESIS-2021.pdf (3.280Mb)
      Date
      2021-06-11
      Author
      Wu, Vicky
      ORCID
      0000-0002-5965-8611
      Type
      Thesis
      Degree Level
      Masters
      Metadata
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      Abstract
      The fermentation redox potential was controlled during the production of 1,3-propanediol (PDO) by Lactobacillus panis PM1. Results show that the best redox potential level was at -200 mV at which had the highest PDO production (23.38 g/L) when compared to that controlled at -250 mV, -150 mV, and without control. For the redox potential level under investigation, the mass PDO yield with respect to glycerol was between 0.66 and 0.82. Potassium ferricyanide was chosen as an oxidant to control redox potential level. With the addition of oxidant, the batch fermentation time was noticeably reduced compared to the one without redox potential control. Furthermore, co-substrate utilization of glucose and glycerol was observed when potassium ferricyanide was present. It was postulated that such co-substrate utilization pattern was resulting from the redox imbalance where the activities of acetaldehyde dehydrogenase and alcohol dehydrogenase were retarded by the presence of potassium ferricyanide. To overcome the redox imbalance, the glycerol-reductive pathway was triggered to serve as the electron source to fuel glycolysis pathway along with the PDO formation. The developed redox potential control strategy may benefit other specifically isolated PDO-producing strains and industrial PDO green processing to further enhance their productivity.
      Degree
      Master of Science (M.Sc.)
      Department
      Chemical and Biological Engineering
      Program
      Chemical Engineering
      Committee
      Zhang, Chris; Meda, Venkatesh; Tanaka, Takuji
      Copyright Date
      May 2021
      URI
      https://hdl.handle.net/10388/13425
      Subject
      Redox potential controlled fermentation, 1,3-propanediol
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