Enhanced methylglyoxal formation in cystathionine γ-lyase knockout mice
| dc.contributor.advisor | Dr. Lingyun Wu | en_US |
| dc.contributor.committeeMember | Dr. Paul Lee | en_US |
| dc.contributor.committeeMember | Dr. Steven Richardson | en_US |
| dc.contributor.committeeMember | Dr. Kaushik Desai | en_US |
| dc.contributor.committeeMember | Dr. Linda Hiebert | en_US |
| dc.contributor.committeeMember | Dr. Thomas Fisher | en_US |
| dc.creator | Untereiner, Ashley Anne | en_US |
| dc.date.accessioned | 2011-05-27T20:36:48Z | en_US |
| dc.date.accessioned | 2013-01-04T04:33:30Z | |
| dc.date.available | 2012-06-24T08:00:00Z | en_US |
| dc.date.available | 2013-01-04T04:33:30Z | |
| dc.date.created | 2011-05 | en_US |
| dc.date.issued | 2011-05-01 | en_US |
| dc.date.submitted | May 2011 | en_US |
| dc.description.abstract | Methylglyoxal (MG) is a reactive glucose metabolite and a known causative factor for hypertension and diabetes. Hydrogen sulfide (H₂S), on the other hand, is a gasotransmitter with multifaceted physiological functions, including anti-oxidant and vasodilatory properties. The present study demonstrates that MG and H₂S can interact with and modulate each other's functions. Upon in vitro incubations, we found that MG and H₂S can directly interact to form three possible MG-H₂S adducts. Furthermore, the endogenous production level of MG or H₂S was significantly reduced in a concentration-dependent manner in rat vascular smooth muscle cells (A-10 cells) treated with NaHS, a H₂S donor, or MG, respectively. Indeed, MG-treated A-10 cells exhibited a concentration-dependent down-regulation of the protein and activity level of cystathionine γ-lyase (CSE), the main H₂S-generating enzyme in the vasculature. Moreover, H₂S can induce the inhibition of MG-generated ROS production in a concentration-dependent manner in A-10 cells. In 6-22 week-old CSE knockout male mice (CSE⁻/⁻), mice with lower levels of vascular H₂S, we observed a significant elevation in MG levels in both plasma and renal extracts. Renal triosephosphates were also significantly increased in the 6-22 week-old CSE⁻/⁻ mice. To identify the source of the elevated renal MG levels, we found that the activity of fructose-1,6-bisphosphatase (FBPase), the rate-limiting enzyme in gluconeogenesis, was significantly down-regulated, along with lower levels of its product (fructose-6-phosphate) and higher levels of its substrate (fructose-1,6-bisphosphate) in the kidney of 6-22 week-old CSE⁻/⁻ mice. We have also observed lower levels of the gluconeogenic regulator, peroxisome proliferator-activated receptor-γ coactivator (PGC)-1α, and its down-stream targets, FBPase-1 and -2, phosphoenolpyruvate carboxykinase (PEPCK), and estrogen-related receptor (ERR)α mRNA expression levels in renal extracts from 6-22 week-old CSE⁻/⁻ mice. Likewise, FBPase-1 and -2 mRNA levels were also significantly down-regulated in aorta tissues from 14-16 week-old CSE⁻/⁻ mice. Administration of 30 and 50 µM NaHS induced a significant increase in FBPase-1 and PGC-1α in rat A-10 cells. We have also observed a significant up-regulation of PEPCK and ERRα mRNA expression levels in 50 µM NaHS-treated A-10 cells, further confirming the involvement of H₂S in regulating the rate of gluconeogenesis and MG formation. Overall, this unique study demonstrates the existence of a negative correlation between MG and H₂S in the vasculature. Further elucidation of this cross-talk phenomenon between MG and H₂S could lead to more elaborate and effective therapeutic regimens to combat metabolic syndrome and its related health complications. | en_US |
| dc.identifier.uri | http://hdl.handle.net/10388/etd-05272011-203648 | en_US |
| dc.language.iso | en_US | en_US |
| dc.subject | Hydrogen sulfide | en_US |
| dc.subject | Fructose-1 6-bisphosphatase | en_US |
| dc.subject | Vascular smooth muscle cells | en_US |
| dc.subject | Peroxisome proliferator-activated receptor-g | en_US |
| dc.subject | Methylglyoxal | en_US |
| dc.subject | Reactive oxygen species | en_US |
| dc.title | Enhanced methylglyoxal formation in cystathionine γ-lyase knockout mice | en_US |
| dc.type.genre | Thesis | en_US |
| dc.type.material | text | en_US |
| thesis.degree.department | Pharmacology | en_US |
| thesis.degree.discipline | Pharmacology | en_US |
| thesis.degree.grantor | University of Saskatchewan | en_US |
| thesis.degree.level | Masters | en_US |
| thesis.degree.name | Master of Science (M.Sc.) | en_US |