Repository logo
 

Immunostimulatory effects and delivery of oligodeoxynucleotides containing CpG motifs (CpG-ODN) in neonatal broiler chickens

dc.contributor.advisorGomis, Susanthaen_US
dc.contributor.committeeMemberMutwiri, Georgeen_US
dc.contributor.committeeMemberGerdts, Volkeren_US
dc.contributor.committeeMemberAllen, Andyen_US
dc.contributor.committeeMemberAllan, Brendaen_US
dc.contributor.committeeMemberVan Kessel, Andrew G.en_US
dc.creatorJoze Taghavi Shirazi, Azitaen_US
dc.date.accessioned2008-04-29T14:16:10Zen_US
dc.date.accessioned2013-01-04T04:30:07Z
dc.date.available-0001-11-30T08:00:00Zen_US
dc.date.available2013-01-04T04:30:07Z
dc.date.created2007en_US
dc.date.issued2007en_US
dc.date.submitted2007en_US
dc.description.abstractOligodeoxynucleotides containing CpG motifs (CpG-ODN) have been shown to stimulate the innate immune system against a variety of bacterial, viral, and protozoan infections in a variety of vertebrate species. The objectives of this study were to investigate the immunostimulatory effect of CpG-ODN against Salmonella Typhimurium infection and the formulation and delivery of CpG-ODN by the in ovo route. Day-old broiler chicks or embryonated eggs (day 18th of incubation) received either 50 µg of CpG-ODN, 50 µg of non-CpG-ODN, or saline. At day four-post hatch, all birds were subcutaneously inoculated by Salmonella Typhimurium. Clinical signs, pathology, bacterial isolations from the air sacs, and mortality were observed for ten days following challenge. The survival rate of the birds that received CpG-ODN via in ovo or in vivo treatments was significantly higher than the control group. Salmonella Typhimurium level in the peripheral blood and pathology were significantly lower (p < 0.001) in CpG-ODN group compared to the control group. In order to investigate the effect of formulation of CpG-ODN, embryonated eggs (day 18th of incubation) were inoculated with either 50µg of CpG-ODN alone or CpG-ODN formulated with polyphosphazene, liposome, or Emulsigen®. Four days after administration of CpG-ODN formulations, the birds were challenged with E. coli by subcutaneous injection. Clinical signs, pathology, bacterial isolations from the air sacs, and mortality were observed for seven days following challenge. The birds that received either CpG-ODN or CpG-ODN formulated with polyphosphazene had significantly higher survival rates (30 and 60%) compared to the birds in groups receiving either non-CpG-ODN or saline. Bacterial loads in the air sacs were lower in groups treated with formulated CpG-ODN compared to the CpG-ODN alone or control groups. However, formulation of CpG-ODN with liposomes or Emulsigen® did not increase the immunoprotective effect against E. coli infection. We showed that treatment with CpG-ODN protects neonatal chickens against an intracellular bacterial infection and that co-treatment of CpG-ODN with polyphosphazene enhances the immunoprotective effect of CpG-ODN.en_US
dc.identifier.urihttp://hdl.handle.net/10388/etd-04292008-141610en_US
dc.language.isoen_USen_US
dc.subjectEmulsigenen_US
dc.subjectAdjuvantsen_US
dc.subjectPolyphosphazenesen_US
dc.subjectLiposomesen_US
dc.subjectImmunostimulatoryen_US
dc.subjectOligodeoxynucleotidesen_US
dc.titleImmunostimulatory effects and delivery of oligodeoxynucleotides containing CpG motifs (CpG-ODN) in neonatal broiler chickensen_US
dc.type.genreThesisen_US
dc.type.materialtexten_US
thesis.degree.departmentVeterinary Pathologyen_US
thesis.degree.disciplineVeterinary Pathologyen_US
thesis.degree.grantorUniversity of Saskatchewanen_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Science (M.Sc.)en_US

Files

Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
azita.pdf
Size:
2.98 MB
Format:
Adobe Portable Document Format
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
905 B
Format:
Plain Text
Description: