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Exploring the dynamics of Salmonella transmission in a murine model of infection

dc.contributor.advisorWhite, Aaron P.en_US
dc.contributor.committeeMemberHoward, Stepehen P.en_US
dc.contributor.committeeMemberKoester, Wolfgang L.en_US
dc.contributor.committeeMemberKorber, Darrenen_US
dc.creatorShivak, Dylanen_US
dc.date.accessioned2015-09-15T12:00:17Z
dc.date.available2015-09-15T12:00:17Z
dc.date.created2015-08en_US
dc.date.issued2015-09-14en_US
dc.date.submittedAugust 2015en_US
dc.description.abstractMost Salmonella enterica serovars are believed to have a cyclical lifestyle involving both host-associated and environment-associated, persistent phases. Their ability to persist in the environment increases the probability that they will be transmitted. Our hypothesis is that the genetic factors required for cellular aggregation and biofilm formation are important for host-to-host transmission. A link between biofilm formation, environmental persistence and transmissibility has not been observed, due to the lack of an appropriate model. We developed a murine model of Salmonella transmission allowing us to study the genetic factors involved in the transmission process. To test the role of aggregation and biofilm formation we used the ∆csgD mutant, which is deficient in both processes. We also engineered luciferase reporter strains of Salmonella enterica serovar Typhimurium (Salmonella Typhimurium) to track infection within a mouse population before the onset of clinical signs using bioluminescent imaging. We determined that mice shed high levels of Salmonella Typhimurium in their feces when pre-treated with streptomycin. To observe the transmission efficiency of Salmonella, we tracked their spread from infected mice to naive mice, and determined that Salmonella could be transmitted only after pre-treatment with streptomycin. We compared the shedding potential and colonization levels of mice challenged with either wild-type Salmonella Typhimurium or the ∆csgD mutant and determined them to be statistically similar when challenged separately. We found that wild-type Salmonella Typhimurium persisted in fecal pellets at higher levels than the ∆csgD mutant. We compared both the short- and long- transmission potential of the ∆csgD mutant to wild type Salmonella Typhimurium, and found that the mutant did not have a defect in either process. Though not observed in our model, we believe that environmental persistence and biofilm formation are important for the transmission of Salmonella due to its cyclical lifestyle. The model we generated remains useful to test the role of other genes in transmission. It can be further refined to more accurately mimic environmental transmission of Salmonella. Further understanding of the transition of Salmonella from infected hosts to the environment and back into new hosts will aid in reducing its environmental persistence and transmission.en_US
dc.identifier.urihttp://hdl.handle.net/10388/ETD-2015-08-2196en_US
dc.language.isoengen_US
dc.subjectSalmonellaen_US
dc.subjecttransmissionen_US
dc.subjectpersistenceen_US
dc.subjectbiofilmen_US
dc.titleExploring the dynamics of Salmonella transmission in a murine model of infectionen_US
dc.type.genreThesisen_US
dc.type.materialtexten_US
thesis.degree.departmentMicrobiology and Immunologyen_US
thesis.degree.disciplineMicrobiology and Immunologyen_US
thesis.degree.grantorUniversity of Saskatchewanen_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Science (M.Sc.)en_US

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