Linking new genes to an odontogenesis TP63-mediated gene regulatory network that is peripheral to jaw morphogenesis
| dc.contributor.advisor | Boughner, Julia | |
| dc.contributor.committeeMember | Krone, Patrick | |
| dc.contributor.committeeMember | Eames, Brian | |
| dc.contributor.committeeMember | Ianowski, Juan | |
| dc.creator | Rostampour, Nasim 1988- | |
| dc.creator.orcid | 0000-0002-6240-7012 | |
| dc.date.accessioned | 2018-02-27T16:11:35Z | |
| dc.date.available | 2018-02-27T16:11:35Z | |
| dc.date.created | 2018-02 | |
| dc.date.issued | 2018-02-27 | |
| dc.date.submitted | February 2018 | |
| dc.date.updated | 2018-02-27T16:11:35Z | |
| dc.description.abstract | Despite that teeth and jawbones can develop and evolve independently of each other, the genetic processes that independently regulate cranial and dental morphogenesis remain unclear. Previous microarray screens indicated that a TP63-mediated gene regulatory network is integral to odontogenesis but peripheral to jaw morphogenesis. Here, I characterized the expression of four genes flagged by our lab’s previous microarray screens, comparing embryonic wild-type and TP63-null (TP63-/-) mutant mouse mandible prominences. I hypothesized that in the normal dental epithelium, TP63 up-regulates Fermt1 and Pltp and down-regulates Cbln1 and Krt8. I validated the expression domains of these genes in TP63-/- mutant and wild-type mice using RNA in-situ hybridization on paraffin tissue sections at two stages, E11.5, just after odontogenesis begins, and at E13.5, just after odontogenesis arrests in the TP63-/- mutant. My in situ results validated our lab’s previous microarray screens. My work revealed that Fermt1 is expressed in wild-type oral epithelium and dental epithelium, and that Pltp is expressed in dental epithelium. Compared to wild-type littermates, in TP63-/- mice, Fermt1 and Pltp expression intensity in the dental epithelium was decreased. Cbln1 was not expressed in wildtype dental epithelium, although it expressed intensely in the dental epithelium of TP63-/- littermates. Krt8 was expressed in the dental epithelium of both wild-type and TP63-/- embryos, with increased intensity in the TP63-/- mice. In sum, my results largely supported my hypothesis. As such, I propose that Fermt1, Pltp, Cbln1, and Krt8 belong to a TP63-mediated gene regulatory network and co-regulate odontogenesis by mediating cell adhesion, cell signaling, and epithelial-mesenchymal interactions at early stages of tooth development. | |
| dc.format.mimetype | application/pdf | |
| dc.identifier.uri | http://hdl.handle.net/10388/8453 | |
| dc.subject | Odontogenesis, TP63, Gene regulatory network | |
| dc.title | Linking new genes to an odontogenesis TP63-mediated gene regulatory network that is peripheral to jaw morphogenesis | |
| dc.type | Thesis | |
| dc.type.material | text | |
| thesis.degree.department | Anatomy and Cell Biology | |
| thesis.degree.discipline | Anatomy and Cell Biology | |
| thesis.degree.grantor | University of Saskatchewan | |
| thesis.degree.level | Masters | |
| thesis.degree.name | Master of Science (M.Sc.) |