The roles of ccf-1 and pal-1 genes in aging and stress resistance of Caenorhabditis elegans
| dc.contributor.advisor | Wu, Michael | |
| dc.contributor.committeeMember | Honaramooz , Ali | |
| dc.contributor.committeeMember | Ferrari, Maud | |
| dc.contributor.committeeMember | Mousseau, Darrell D | |
| dc.creator | Tabarraei, Hadi | |
| dc.creator.orcid | 0000-0002-6296-9672 | |
| dc.date.accessioned | 2023-10-17T21:55:21Z | |
| dc.date.available | 2023-10-17T21:55:21Z | |
| dc.date.copyright | 2023 | |
| dc.date.created | 2023-10 | |
| dc.date.issued | 2023-10-17 | |
| dc.date.submitted | October 2023 | |
| dc.date.updated | 2023-10-17T21:55:22Z | |
| dc.description.abstract | The CCR4-NOT complex is a critical regulator of gene expression involved in multiple cellular processes, including transcriptional regulation, RNA metabolism, and stress responses. In this thesis, I investigated the role of ccf-1, a component of the CCR4-NOT complex, in stress responses and its necessity for normal lifespan and its implications in various pro-longevity signaling pathways in C. elegans. Through my experiments using environmental stressors such as cadmium and acrylamide, I measured several stress-responsive gene expression levels and observed that ccf-1 is required for stress-induced transcriptional changes in the model organism Caenorhabditis elegans. Additionally, I found that the ccf-1 gene plays an important role in the lifespan of several long-lived mutants, highlighting its significance in the aging process. I also showed that other components of the CCR4-NOT complex are also involved in promoting proper transcriptional response to environmental stressors. Furthermore, my research led to the discovery of the PAL-1 protein as a novel transcriptional factor involved in stress response with pleiotropic effects, demonstrating additive impacts when interacting with the ccf-1 gene. These findings contribute to a better understanding of the molecular mechanisms governing stress responses and aging in C. elegans and may have broader implications in unraveling similar processes in higher organisms, including humans. | |
| dc.format.mimetype | application/pdf | |
| dc.identifier.uri | https://hdl.handle.net/10388/15144 | |
| dc.language.iso | en | |
| dc.subject | Aging | |
| dc.subject | CCR4-NOT complex | |
| dc.subject | stress response | |
| dc.subject | Caenorhabditis elegans | |
| dc.title | The roles of ccf-1 and pal-1 genes in aging and stress resistance of Caenorhabditis elegans | |
| dc.type | Thesis | |
| dc.type.material | text | |
| thesis.degree.department | Veterinary Biomedical Sciences | |
| thesis.degree.discipline | Veterinary Biomedical Sciences | |
| thesis.degree.grantor | University of Saskatchewan | |
| thesis.degree.level | Masters | |
| thesis.degree.name | Master of Science (M.Sc.) |