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One-carbon (C-1) metabolism in response to biotic and abiotic stresses

dc.contributor.advisorKing, Johnen_US
dc.contributor.committeeMemberWei, Yangdouen_US
dc.contributor.committeeMemberSelvaraj, Gopalanen_US
dc.contributor.committeeMemberMessier, Françoisen_US
dc.contributor.committeeMemberGray, Gordon R.en_US
dc.creatorLiu, Weipingen_US
dc.date.accessioned2005-03-16T18:25:18Zen_US
dc.date.accessioned2013-01-04T04:26:51Z
dc.date.available2005-03-17T08:00:00Zen_US
dc.date.available2013-01-04T04:26:51Z
dc.date.created2005-02en_US
dc.date.issued2005-02-15en_US
dc.date.submittedFebruary 2005en_US
dc.description.abstractIn plants, the generation and supply of methyl units is important in one-carbon (C-1) metabolism, which is essential to all organisms. I have identified a series of cDNA sequences encoding N5, N10-methylenetetrahydrofolate reductase (MTHFR), cobalamin-independent methionine synthase (Met Syn), S-adenosylmethionine synthetase (isoform I, AdoMet Syn2661 and isoform II, AdoMet Syn605), S-adenosylmethionine decarboxylase (SAMDC), serine hydroxymethyltransferase (SHMT) and N5, N10-methenyltetrahydrofolate cyclohydrolase / N5, N10-methylenetetrahydrofolate dehydrogenase (THFC/THFD) in the pathways of generation and supply of methyl units. These are from a cDNA library of mRNA from a susceptible wheat (Triticum monococcum) (Tm) line 441 epidermis, 24 h after inoculation with powdery mildew fungus (Blumeria graminis f. sp. tritici) (Bgt). Phylogenetic tree cluster analysis and subcellular localization prediction by TargetP revealed that MTHFR, Met Syn, AdoMet Syn605, AdoMet Syn2661, SAMDC, and THFC/THFD may be localized in cytosol; SHMT may be localized in mitochondria. Northern blot analysis indicated that expression of MTHFR, Met Syn, AdoMet Syn2661, AdoMet Syn605 and SAMDC genes was up-regulated by powdery mildew infection, abiotic stresses and treatments with stress signal molecules; expression of SHMT and THFC/THFD was either constitutive or down-regulated. These results suggest a close metabolic link between various stresses and the pathways of generation and supply of methyl units in this wheat.en_US
dc.identifier.urihttp://hdl.handle.net/10388/etd-03162005-182518en_US
dc.language.isoen_USen_US
dc.subjectone-carbonen_US
dc.subjectbiotic stressen_US
dc.subjectabiotic stressen_US
dc.subjectmetabolismen_US
dc.titleOne-carbon (C-1) metabolism in response to biotic and abiotic stressesen_US
dc.type.genreThesisen_US
dc.type.materialtexten_US
thesis.degree.departmentBiologyen_US
thesis.degree.disciplineBiologyen_US
thesis.degree.grantorUniversity of Saskatchewanen_US
thesis.degree.levelMastersen_US
thesis.degree.nameMaster of Science (M.Sc.)en_US

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