Identifying new protocol methods in polyploidy research with Cannabis sativa L
The development and critique of an improved experiment and protocol analysis is critical in terms of this research experiment. With little published research in the development of polyploid cannabis, it was crucial that steps were taken to test and report the methods and protocols provided thus far. The development and discovery that Pisum sativum L. impeded the ploidy analysis of Cannabis sativa L. in flow cytometry when used as an internal standard was recognized. In turn, these findings only reinforced the need to find and test better suited endogenous controls for Cannabis sativa L. samples within flow cytometry analysis. The works of producing a more suited protocol for Cannabis sativa L. flow cytometry we can better understand the effects of possible secondary metabolites between the plants within flow cytometry as well as help pave the way for future Cannabis sativa L. ploidy research.
cannabis, polyploid cannabis, protocol methods
Bachelor of Arts and Science (B.A.&Sc.)