RAGE-DEPENDENT SENSITIZATION OF THORACIC SENSORY NEURONS DURING INFLAMMATORY CONDITIONS
Date
2016-12-19
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
ORCID
Type
Thesis
Degree Level
Masters
Abstract
Various airway inflammatory diseases progress as a result of chronic inflammation, specifically neurogenic inflammation. One factor known to be involved in this is RAGE. This work has established RAGE as a key mediator in LPS-induced sensitization of afferent thoracic dorsal root ganglia neurons of the upper airway in mice. This was demonstrated from electrophysiological data showing the failure of LPS to potentiate capsaicin-evoked TRPV1 currents and increase action potential generation in RAGE KO relative to wild-type thoracic neurons. Mass spectrometry analysis for protein identification represents a step forward in biochemistry, allowing to obtain a global picture of the proteins expressed in a cell, including isoforms, post-translational modifications and downstream signalling pathways.
Description
Keywords
RAGE, RAGE isoforms, RAGE KO, thoracic dorsal root ganglia, LPS, airway inflammatory disease, neurogenic inflammation, sensory neuron sensitization
Citation
Degree
Master of Science (M.Sc.)
Department
Physiology
Program
Physiology